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1

Kinetic Studies of the Effect of pH on the Trypsin-Catalyzed Hydrolysis of N -Î±-benzyloxycarbonyl- l -lysine- p -nitroanilide: Mechanism of Trypsin Catalysis

Malthouse, J. Paul G.

Journal:

Year:

2020

Language:

english

File:

PDF, 2.03 MB

english, 2020

2

Enzymatic synthesis of isotopically labelled serine and tryptophan for application in peptide synthesis

J. Paul G. Malthouse, Teresa B. Fitzpatrick, John J. Milne, Leif Grehn, Ulf Ragnarsson

Journal:

Journal of Peptide Science

Year:

1997

Language:

english

File:

PDF, 102 KB

english, 1997

3

13C nuclear magnetic resonance observations on the interaction between p-amidinophenylpyruvic acid and trypsin

William U. Primrose, Neil E. Mackenzie, J.Paul G. Malthouse, A.Ian Scott

Journal:

Bioorganic Chemistry

Year:

1985

Language:

english

File:

PDF, 528 KB

english, 1985

4

Conformational, receptor interaction and alanine scan studies of glucose-dependent insulinotropic polypeptide

Kalyana C. Venneti, J. Paul G. Malthouse, Finbarr P.M. O'Harte, Chandralal M. Hewage

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2011

Language:

english

File:

PDF, 1.02 MB

english, 2011

5

pH stability of the stromelysin-1 catalytic domain and its mechanism of interaction with a glyoxal inhibitor

Nicole Howe, Mariangela Ceruso, Edward Spink, J. Paul G. Malthouse

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2011

Language:

english

File:

PDF, 1.03 MB

english, 2011

6

Ionisations within a subtilisin–glyoxal inhibitor complex

Aleksandra Djurdjevic-Pahl, Chandralal Hewage, J. Paul G. Malthouse

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2005

Language:

english

File:

PDF, 319 KB

english, 2005

7

Oxyanion and tetrahedral intermediate stabilisation by subtilisin: Detection of a new tetrahedral adduct

Nicole Howe, Louis Rogers, Chandralal Hewage, J. Paul G. Malthouse

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2009

Language:

english

File:

PDF, 464 KB

english, 2009

8

Stereospecificity of α-proton exchange reactions catalysed by pyridoxal-5′-phosphate-dependent enzymes

J.Paul G. Malthouse

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2003

Language:

english

File:

PDF, 110 KB

english, 2003

9

The bioactive conformation of glucose-dependent insulinotropic polypeptide by NMR and CD spectroscopy

Iñigo Alaña, J. Paul G. Malthouse, Finbarr P. M. O'Harte, Chandralal M. Hewage

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2007

Language:

english

File:

PDF, 419 KB

english, 2007

10

Hyperfine coupling of 17O to Mo(V) in the rapid epr signal of xanthine oxidase: Evidence for an oxygen ligand of the metal

Steven Gutteridge, J.Paul G. Malthouse, Robert C. Bray

Journal:

Journal of Inorganic Biochemistry

Year:

1979

Language:

english

File:

PDF, 449 KB

english, 1979

11

The effect of histidine-228 on the catalytic efficiency and stereospecificity of the serine hydroxymethyltransferase catalysed exchange of the α-protons of amino acids

Teresa B. Fitzpatrick, J.Paul G. Malthouse

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Year:

1998

Language:

english

File:

PDF, 212 KB

english, 1998

12

The aspartate aminotransferase-catalysed exchange of the α-protons of aspartate and glutamate: the effects of the R386A and R292V mutations on this exchange reaction

Marrita M Mahon, Rachel Graber, Philipp Christen, J.Paul G Malthouse

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Year:

1999

Language:

english

File:

PDF, 281 KB

english, 1999

13

A substrate-induced change in the stereospecificity of the serine-hydroxymethyltransferase-catalysed exchange of the α-protons of amino acids : Evidence for a second catalytic site

Teresa B. Fitzpatrick, J. Paul G. Malthouse

Journal:

Year:

1998

Language:

english

File:

PDF, 146 KB

english, 1998

14

Characterization of the Electrostatic Perturbation of a Catalytic Site (Cys)-S–/(His)-Im+H Ion-pair in One Type of Serine Proteinase Architecture by Kinetic and Computational Studies on Chemically Mutated Subtilisin Variants

Francisco J. Plou, Devanand Kowlessur, Paul G.J. Malthouse, Geoffrey W. Mellor, Michael J. Hartshorn, Surapong Pinitglang, Hasu Patel, Christopher M. Topham, Emrys W. Thomas, Chandra Verma, Keith Broc

Journal:

Journal of Molecular Biology

Year:

1996

Language:

english

File:

PDF, 1012 KB

english, 1996

15

The case for assigning a value of approximately 4 to pKaI of the essential histidine-cysteine interactive systems of papain, bromelain and ficin

Michael Shipton, Marek P.J. Kierstan, J. Paul G. Malthouse, Trevor Stuchbury, Keith Brocklehurst

Journal:

Year:

1975

Language:

english

File:

PDF, 392 KB

english, 1975

16

NMR structure of the glucose-dependent insulinotropic polypeptide fragment, GIP(1–30)amide

Iñigo Alaña, Chandralal M. Hewage, J. Paul G. Malthouse, Jeremy C. Parker, Victor A. Gault, Finbarr P.M. O'Harte

Journal:

Biochemical and Biophysical Research Communications

Year:

2004

Language:

english

File:

PDF, 412 KB

english, 2004

17

Gliotoxins disrupt alanine metabolism and glutathione production in C6 glioma cells: a 13C NMR spectroscopic study

Lorraine Brennan, Chandralal Hewage, J. Paul G. Malthouse, Gethin J. McBean

Journal:

Neurochemistry International

Year:

2004

Language:

english

File:

PDF, 240 KB

english, 2004

18

Impact of the gliotoxin l-serine-O-sulphate on cellular metabolism in cultured rat astrocytes

Lorraine Brennan, Paula M. Alves, Chandralal Hewage, J. Paul G. Malthouse, Gethin J. McBean

Journal:

Neurochemistry International

Year:

2006

Language:

english

File:

PDF, 283 KB

english, 2006

19

The pyridoxal-5′-phosphate-dependent catalytic antibody 15A9: its efficiency and stereospecificity in catalysing the exchange of the α-protons of glycine

Marrita M. Mahon, Svetlana I. Gramatikova, Philipp Christen, Teresa B. Fitzpatrick, J.Paul G. Malthouse

Journal:

Year:

1998

Language:

english

File:

PDF, 124 KB

english, 1998

20

An NMR study of alterations in [1-13C]glucose metabolism in C6 glioma cells by gliotoxic amino acids

Lorraine Brennan, Chandralal Hewage, J.Paul G. Malthouse, Gethin J. McBean

Journal:

Neurochemistry International

Year:

2003

Language:

english

File:

PDF, 137 KB

english, 2003

21

Mechanism of the binding of Z–L-tryptophan and Z–L-phenylalanine to thermolysin and stromelysin-1 in aqueous solutions

Mariangela Ceruso, Nicole Howe, J. Paul G. Malthouse

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2012

Language:

english

File:

PDF, 676 KB

english, 2012

22

Marine Connectivity in a Large Inverse Estuary

Kämpf, Jochen, Payne, Nicholas, Malthouse, Paul

Journal:

Journal of Coastal Research

Year:

2010

Language:

english

File:

PDF, 849 KB

english, 2010

23

pH-Dependent Spectroscopic Changes Associated with the Hydroquinone of FMN in Flavodoxins †

Yalloway, Gary N., Mayhew, Stephen G., Malthouse, J. Paul G., Gallagher, Mary E., Curley, G. Paul

Journal:

Year:

1999

Language:

english

File:

PDF, 116 KB

english, 1999

24

Vinyl Sulfone-Based Peptidomimetics as Anti-Trypanosomal Agents: Design, Synthesis, Biological and Computational Evaluation

Dunny, Elizabeth, Doherty, William, Evans, Paul, Malthouse, J. Paul G., Nolan, Derek, Knox, Andrew J. S.

Journal:

Journal of Medicinal Chemistry

Year:

2013

Language:

english

File:

PDF, 1.00 MB

english, 2013

25

Hemiacetal stabilization in a chymotrypsin inhibitor complex and the reactivity of the hydroxyl group of the catalytic serine residue of chymotrypsin

Cleary, Jennifer A., Doherty, William, Evans, Paul, Malthouse, J.Paul G.

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2014

Language:

english

File:

PDF, 1.66 MB

english, 2014

26

Carbon-13 NMR of cyanylated flavodoxin from Megasphaera elsdenii and of thiocyanate model compounds

Doherty, Gerald M., Motherway, Rosemarie, Mayhew, Stephen G., Malthouse, J. Paul G.

Journal:

Year:

1992

Language:

english

File:

PDF, 1.14 MB

english, 1992

27

13 C NMR Study of How the Oxyanion p K a Values of Subtilisin and Chymotrypsin Tetrahedral Adducts Are Affected by Different Amino Acid Residues Binding in Enzyme Subsites S 1 −S 4†,‡

O'Sullivan, Denis B., O'Connell, Timothy P., Mahon, Marrita M., Koenig, Andreas, Milne, John J., Fitzpatrick, Teresa B., Malthouse, J. Paul G.

Journal:

Year:

1999

Language:

english

File:

PDF, 126 KB

english, 1999

28

Detection of a tetrahedral adduct in a trypsin-chloromethyl ketone specific inhibitor complex by carbon-13 NMR

Malthouse, J. Paul G., Mackenzie, Neil E., Boyd, Alan S. F., Scott, A. Ian

Journal:

Journal of the American Chemical Society

Year:

1983

Language:

english

File:

PDF, 250 KB

english, 1983

29

Determination of the Structure of Tetrahedral Transition State Analogues Bound at the Active Site of Chymotrypsin Using 18 O and 2 H Isotope Shifts in the 13 C NMR Spectra of Glyoxal Inhibitors †

Spink, Edward, Hewage, Chandralal, Malthouse, J. Paul G.

Journal:

Year:

2007

Language:

english

File:

PDF, 111 KB

english, 2007

30

Importance of Tetrahedral Intermediate Formation in the Catalytic Mechanism of the Serine Proteases Chymotrypsin and Subtilisin

Petrillo, Teodolinda, O’Donohoe, Catrina A., Howe, Nicole, Malthouse, J. Paul G.

Journal:

Year:

2012

Language:

english

File:

PDF, 443 KB

english, 2012

31

Carbon-13 NMR study of the ionizations within a trypsin-chloromethyl ketone inhibitor complex

Malthouse, J. Paul G., Primrose, William U., Mackenzie, Neil E., Scott, A. Ian

Journal:

Year:

1985

Language:

english

File:

PDF, 1.08 MB

english, 1985

32

Structure and stereochemistry of tetrahedral inhibitor complexes of papain by direct NMR observation

Gamcsik, Michael P., Malthouse, J. Paul G., Primrose, William U., Mackenzie, Neil E., Boyd, Alan S. F., Russell, Richard A., Scott, A. Ian

Journal:

Journal of the American Chemical Society

Year:

1983

Language:

english

File:

PDF, 266 KB

english, 1983

33

Claisen-Type Addition of Glycine to Pyridoxal in Water

Toth, Krisztina, Amyes, Tina L., Richard, John P., Malthouse, J. Paul G., NíBeilliú, Máire E.

Journal:

Journal of the American Chemical Society

Year:

2004

Language:

english

File:

PDF, 50 KB

english, 2004

34

Cryoenzymology of proteases: NMR detection of a productive thioacyl derivative of papain at subzero temperatures

Malthouse, J. Paul G., Gamcsik, Michael P., Boyd, Alan S. F., Mackenzie, Neil E., Scott, A. Ian

Journal:

Journal of the American Chemical Society

Year:

1982

Language:

english

File:

PDF, 400 KB

english, 1982

35

Carbon-13 NMR study of the stereospecificity of the thiohemiacetals formed on inhibition of papain by specific enantiomeric aldehydes

Mackenzie, Neil E., Grant, Stephan K., Scott, A. Ian, Malthouse, J. Paul G.

Journal:

Year:

1986

Language:

english

File:

PDF, 705 KB

english, 1986

36

A 13 C-NMR study of azacryptand complexes

Wild, Aljoscha A. C., Fennell, Kevin, Morgan, Grace G., Hewage, Chandralal M., Malthouse, J. Paul G.

Journal:

Dalton Transactions

Year:

2014

Language:

english

File:

PDF, 601 KB

english, 2014

37

Quantifying tetrahedral adduct formation and stabilization in the cysteine and the serine proteases

Cleary, Jennifer A., Doherty, William, Evans, Paul, Malthouse, J. Paul G.

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2015

Language:

english

File:

PDF, 1013 KB

english, 2015

38

Structural and biochemical properties of LuxF from Photobacterium leiognathi

Bergner, Thomas, Tabib, Chaitanya R., Winkler, Andreas, Stipsits, Steve, Kayer, Heidemarie, Lee, John, Malthouse, J. Paul, Mayhew, Stephen, Müller, Franz, Gruber, Karl, Macheroux, Peter

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2015

Language:

english

File:

PDF, 3.46 MB

english, 2015

39

NMR Study of the Inhibition of Pepsin by Glyoxal Inhibitors: Mechanism of Tetrahedral Intermediate Stabilization by the Aspartyl Proteases †

Cosgrove, Sonya, Rogers, Louis, Hewage, Chandralal M., Malthouse, J. Paul G.

Journal:

Year:

2007

Language:

english

File:

PDF, 220 KB

english, 2007

40

Marine Connectivity in a Large Inverse Estuary

Jochen Kämpf, Nicholas Payne and Paul Malthouse

Journal:

Journal of Coastal Research

Year:

2010

Language:

english

File:

PDF, 2.70 MB

english, 2010

41

The stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the α-protons of amino acids

NíBEILLIÚ, Máire E., MALTHOUSE, J. Paul G.

Journal:

Biochemical Journal

Year:

2004

Language:

english

File:

PDF, 235 KB

english, 2004

42

A new lysine derived glyoxal inhibitor of trypsin, its properties and utilization for studying the stabilization of tetrahedral adducts by trypsin

Cleary, Jennifer A., Malthouse, J. Paul G.

Journal:

Biochemistry and Biophysics Reports

Year:

2016

Language:

english

File:

PDF, 2.60 MB

english, 2016

43

MP74-07 POST-VASECTOMY SEMEN ANALYSIS OPTIMAL TIMING AND FINANCIAL IMPLICATIONS OF REPEAT TESTING

Kasivisvanathan, Veeru, Nehikhare, Osayuki, Kadirvelarasan, Ahayla, Ali, Yasir, Golban, Nathan, Kulendran, Sinthu, Malthouse, Theo, Ali, Yousif, Challacombe, Ben, Hegarty, Paul, Shabbir, Majed

Journal:

The Journal of Urology

Year:

2015

Language:

english

File:

PDF, 274 KB

english, 2015

44

A 13 C-NMR study of the role of Asn-155 in stabilizing the oxyanion of a subtilisin tetrahedral adduct

O'CONNELL, Timothy P., DAY, Regina M., TORCHILIN, Ekaterina V., BACHOVCHIN, William W., MALTHOUSE, J. Paul G.

Journal:

Biochemical Journal

Year:

1997

Language:

english

File:

PDF, 409 KB

english, 1997

45

A study of the tryptophan synthase catalysed H/D exchange of the α-protons of amino acids

MILNE, JOHN J., MALTHOUSE, J. PAUL G.

Journal:

Biochemical Society Transactions

Year:

1994

Language:

english

File:

PDF, 107 KB

english, 1994

46

A study of the pH dependence of the reaction of benzyloxycarbonyltryptophanylchloromethane with chymotrypsin

Mahon, Marrita M., Malthouse, J. Paul G.

Journal:

Biochemical Society Transactions

Year:

1996

Language:

english

File:

PDF, 121 KB

english, 1996

47

Enzymatic synthesis of α-deuterated amino acids

Milne, John J., Malthouse, J. Paul G.

Journal:

Biochemical Society Transactions

Year:

1996

Language:

english

File:

PDF, 121 KB

english, 1996

48

13C-NMR study of the inhibition of δ-chymotrypsin by a tripeptide-glyoxal inhibitor

DJURDJEVIC-PAHL, Aleksandra, HEWAGE, Chandralal, MALTHOUSE, J. Paul G.

Journal:

Biochemical Journal

Year:

2002

Language:

english

File:

PDF, 203 KB

english, 2002

49

Evidence that binding to the S 2 -subsite of papain may be coupled with catalytically relevant structural change involving the cysteine-25–histidine-159 diad. Kinetics of the reaction of papain with a two-protonic-state reactivity probe containing a hydrophobic side chain

Brocklehurst, Keith, Malthouse, J. Paul G., Shipton, Michael

Journal:

Biochemical Journal

Year:

1979

Language:

english

File:

PDF, 1.27 MB

english, 1979

50

A 13C-NMR study of the inhibition of papain by a dipeptide-glyoxal inhibitor

LOWTHER, Jonathan, DJURDJEVIC-PAHL, Aleksandra, HEWAGE, Chandralal, MALTHOUSE, J. Paul G.

Journal:

Biochemical Journal

Year:

2002

Language:

english

File:

PDF, 120 KB

english, 2002

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